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Thermo Fisher
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Journal: Chemical Science
Article Title: SpectraSage unveils specific proteolytic patterns of 20S on mono-ubiquitylated Tau proteoforms involved in neurodegeneration
doi: 10.1039/d5sc04240b
Figure Lengend Snippet: Sequence coverage and distribution patterns of peptides of Ub (A) and tauK18 (B) identified in both TMT labelled and tryptic digested samples following 3 h of incubation of Ub-tau254 with h20S proteasome. Peptide identification confidence is visually represented by color. Green peptides exhibit high confidence while blue peptides indicate medium confidence. TMTproZero labels are indicated by a purple circle on peptide N-termini and a purple background on Lys residues. The -GG remnant on Cys side chains is marked by a C with a dot on top. Inset: frequency distribution of SequestHT XCorr values, binned into ranges ( x -axis: 1–2, 2–3, 3–4, >4), showing the distribution of Ub (green) and tau (red) peptides with and without trypsin treatment. A comprehensive list of peptides is provided in SI Data 1 and 2. (C) Peptide Spectrum Matches (PSMs) of selected Ub peptides.
Article Snippet: Eluted peptides were then labelled by
Techniques: Sequencing, Incubation